Pore-forming proteins and host-pathogen interactions

Saibil group, ISMB
Birkbeck College London
pleurotolysin pore

X-ray tomogram
Hale, VL, Watermeyer, JM, Hackett, F, Vizcay-Barrena, G, van Ooij, C, Thomas, JA, Spink, MC, Harkiolaki, M, Duke, E, Fleck, RA, Blackman, MJ & Saibil, HR (2017) Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress. PNAS 114, 3439-3444.
AL BRMUE AL BRMUE
  • Leung,
  • C, Hodel,
  • AW, Brennan,
  • AJ,  Lukoyanova, N, Tran,S, House,
  • CM, Kondos,
  • SC, Whisstock,
  • JC, Dunstone,
  • MD, Trapani, JA,  Voskoboinik,
  • I, Saibil, HR & Hoogenboom, BW (2017) Real-time visualization of perforin nanopore assembly. Nature Nanotechnol. doi:10.1038/nnano.2016.303
C9 pore
Watermeyer, JM, Hale, VL, Hackett, F, Clare, DK, Cutts, EE, Vakonakis, I, Fleck, RA, Blackman, MJ and Saibil, HR (2016) A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes. Blood 127, 343-351.
C9 pore
Dudkina, NV, Spicer, BA, Reboul, CF, Conroy, PJ, Lukoyanova, N, Elmlund, H, Law, RHP, Ekkel, SM, Kondos, SC, Goode, RJA, Ramm, G, Whisstock, JC, Saibil, HR, Dunstone, MA (2016) Structure of the poly-C9 component of the complement Membrane Attack Complex. Nature Comm. 7:10588.
Pleurotolysin
              pore
Conformational changes during pore formation by the perforin-related protein pleurotolysin. Lukoyanova, N, Kondos, SC, Farabella, I, Law, RHP, Reboul, CF, Caradoc-Davies, TT, Spicer, BA, Kleifeld, O, Traore, D, Ekkel, SM, Voskoboinik, I, Trapani, JA, Hatfaludi, T, Oliver, K, Hotze, EM, Tweten, RK, Whisstock, JC, Topf, M, Saibil, HR & Dunstone, MA (2015) PLoS Biol. 13:e1002049
T3SS-host
Structure of a bacterial type III secretion system in contact with a host membrane in situ. Nans, A, Kudryashev, M, Saibil, HR & Hayward, RD (2015) Nature Commun. 6:10114.
Suilysin model
Stepwise visualization of membrane pore formation by suilysin, a bacterial cholesterol-dependent cytolysin. Leung, C, Dudkina, NV, Lukoyanova, N, Hodel, AW, Farabella, I, Pandurangan, AP, Jahan, N, Damaso, MP, Osmanovic, D, Reboul, CF, Dunstone, MA, Andrew, PW, Lonnen, R, Topf, M, Saibil, HR & Hoogenboom, BW (2014) eLife 3: e04247.
Cell Micro cover
Pathogen-host reorganisation during Chlamydia invasion revealed by cryo-electron tomography. Nans, A, Saibil, HR & Hayward, R (2014) Cell. Microbiol. 16, 1457-1472.
Traffic cover
Chlamydia assemble a pathogen synapse to hijack the host endoplasmic reticulum. Dumoux, M, Clare, DK, Saibil, HR & Hayward, R (2012) Traffic 13, 1612-1627.
perforin pore
Structural basis for membrane binding and pore formation by lymphocyte perforin. Law, R, Lukoyanova, N, Voskoboinik, I Caradoc-Davies, TT, Baran, K, Dunstone, MA, D'Angelo, ME, Orlova, EV, Coulibaly, F, Verschoor, S, Browne, KA, Ciccone, A, Kuiper, MJ, Bird, PI, Trapani, JA, Saibil, HR & Whisstock, JC (2010) Nature 468, 447-451.
MACPF domain
Friend or foe: the same fold for attack and defense. Lukoyanova, N & Saibil, HR (2008) Trends Immunol. 29, 51-53.
pneumolysin
Structural basis of pore formation by the bacterial toxin pneumolysin. Tilley, SJ, Orlova, EV, Gilbert, RJC, Andrew, PW & Saibil, HR (2005) Cell 121, 247-256.

Birkbeck EM group
Helen Saibil