Electron microscopy and image processing
Institute of Structural and Molecular Biology
Birkbeck College and University College London
|EM Lab 020
T12 Microscope ext 8057
T10 Microscope ext 8058
Polara 0203 0738 090
EM lab safety
Local EM information
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Polara machines, with a K2 direct electron detector and Quantum energy filter on the Polara, DE20 direct detectors on the Polara and F20, and a vitrobot for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.
A Krios g3i 300 kV cryo microscope is currently being assembled, equipped with Falcon 3, K2 Quantum energy filter (to be ugraded to K3) and phase plate.
Photos of a challenging Krios delivery
Hardware: Linux clusters and graphics workstations
Software: Spider, Imagic, MRC image programs, Relion, CCP4, EMAN, Amira, IMOD, Dynamo, Cryosparc
Spider Imagic CCP4 CCP-EM EMAN IMOD Dynamo RELION Cryosparc
eBIC: electron Bio-Imaging Centre at Diamond Light Source