Electron microscopy and image processing
Institute of Structural and Molecular Biology
Birkbeck College and University College London
Facility Coordinator Dr Shu Chen s.chen [at] bbk.ac.uk |
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EM lab safety EM Wiki Local EM information Booking system |
EM groups
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EM equipment
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Krios machines, with Falcon 3 and K3 direct electron detectors and BioQuantum energy filter and phase plate on the Krios, a DE20 direct detector on the F20, and a Vitrobot and Leica Gp2 for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.
Photos of a challenging Krios delivery
Image processing software:
RELION Cryosparc CCP-EM Amira CCP4 EMAN IMOD Dynamo CryoDRGN
eBIC: electron Bio-Imaging Centre at Diamond Light Source