Electron microscopy and image processing

Institute of Structural and Molecular Biology
Birkbeck College and University College London

EM Lab 020 7079 8061
T12 Microscope ext 8057

T10 Microscope ext 8058
Polara 0203 0738 090 
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EM lab safety


EM Wiki


Local EM information


Booking system

Natasha Lukoyanova:
High resolution EM coordinator

Academic management
Carolyn Moores

EM groups

Giulia Zanetti
Anthony Roberts
Cara Vaughan
Finn Werner

Shu Chen:
EM lab manager


Helen Saibil


Elena Orlova
Maya Topf

Gabriel Waksman
Alan Cheung

EMBO
                course

Course Files 2015
Recent course: 5-15 Sept 2017
       

EM equipment
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Polara machines, with a K2 direct electron detector and Quantum energy filter on the Polara, DE20 direct detectors on the Polara and F20, and a vitrobot for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.
Coming in 2017:  a state of the art, 300 kV cryo microscope funded by Wellcome Trust, Birkbeck and UCL.

Image processing 
Hardware: Linux clusters and graphics workstations
Software: Spider, Imagic, MRC image programs, Relion, CCP4, EMAN, Amira, IMOD, Dynamo
Spider Imagic CCP4 CCP-EM EMAN IMOD RELION
Dynamo

eBIC: electron Bio-Imaging Centre at Diamond Light Source