Electron microscopy and image processing

Institute of Structural and Molecular Biology
Birkbeck College and University College London

EM Lab 020 7079 8061
T12 Microscope ext 8057

T10 Microscope ext 8058
Polara 0203 0738 090 

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EM lab safety

EM Wiki

Local EM information

Booking system

Natasha Lukoyanova:
High resolution EM coordinator

Academic management
Carolyn Moores

EM groups

Giulia Zanetti
Anthony Roberts
Cara Vaughan
Finn Werner

Shu Chen:
EM lab manager

Helen Saibil

Elena Orlova
Maya Topf

Gabriel Waksman
Alan Cheung


Course Files 2017

EM equipment
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Polara machines, with a K2 direct electron detector and Quantum energy filter on the Polara, DE20 direct detectors on the Polara and F20, and a vitrobot for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.
A Krios g3i 300 kV cryo microscope is currently being assembled, equipped with Falcon 3, K2 Quantum energy filter (to be ugraded to K3) and phase plate.

Photos of a challenging Krios delivery

Image processing 
Hardware: Linux clusters and graphics workstations
Software: Spider, Imagic, MRC image programs, Relion, CCP4, EMAN, Amira, IMOD, Dynamo, Cryosparc

eBIC: electron Bio-Imaging Centre at Diamond Light Source