| VirBX Brucella suis | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| pVirBsuis | pUC | Vir operon | |
| VirB1 | pET101 | 1-239* | full length C-ter His6 |
| virB4 | pT7- His6Trx / pET32 | 1-831* | full length |
| virB4 | pT7- His6Trx / pET32 | 1-831* | full length without STOP |
| virB4 | pET151 | 1-831* | full length |
| virB4 | pCDF-Duet-1 | 1-831* | full length |
| VirB4 | pET151 | 31-373 | domain N |
| VirB4 | pET151 | 401-800 | domain C |
| VirB4 | pET151 | 31-800 | domain NC |
| virB4 | pT7-7strep | 1-831* | full length |
| VirB4 | pT7-7strep | 1-831* | full length |
| virB5 | pT7-7strep | 18-236 | lacking signal seq. aa18- |
| VirB5 | pET101 | 1-236* | full length C-ter His6 |
| VirB5 | pASK-IBA3C | 1-236* | full length C-ter StrepII |
| VirB6 | pTrc200 | 1-347* | full length |
| VirB6 | pET151 | 260-347 | cytoplasmic fragme |
| VirB6 | pCDF | 260-347 | full length C-ter His6 |
| VirB7 | pET101 | 17-58 | lacking signal seq. aa17-- |
| virB7 | pT7- His6Trx | 17-58 | lacking signal seq. aa17-- |
| virB8 | pT7-7strep | cytoplasmic fragment | |
| VirB8 | pET151 | 1-46 | cytoplasmic fragment |
| VirB8 | pCDF | 1-46 | lacking signal seq. aa19- |
| virB9 | pET151 | 19-289 | lacking signal seq. aa19- |
| virB9 | pASK-4 | 19-147 | lacking signal seq. aa19- |
| virB9 | pASK-4 | 154-289 | lacking signal seq. aa19- |
| virB9 | pASK-4 | 19-289 | lacking signal seq. aa19- |
| virB9 | pT7-7strep | 19-289 | lacking signal seq. aa19- |
| virB9 | pET151 | 19-147 | lacking signal seq. aa19- |
| virB9 | pET151 | 165-289 | lacking signal seq. aa19- |
| virB9 | pET151 | 172-289 | lacking signal seq. aa19- |
| virB9 | pET151 | 165-283 | lacking signal seq. aa19- |
| virB9 | pET151 | 172-283 | lacking signal seq. aa19- |
| virB10 | pT7-7strep | lacking N-terminus aa53- | |
| VirB10 | pET151 | 1-33 | cytoplasmic fragment |
| VirB10 | pCDF | 1-33 | cytoplasmic fragment |
| virB11 | pT7-7strep | full length | |
| virB11 | pCDF | 1-361* | full length |
| VirB8 | pRSF-Duet1 | 1-240 | full length N-ter His6 |
| StrepII-VirB4 f.l. + VirB8 f.l. | pT7-7strep | 1-831 and 1-240 | |
| StrepII-VirB4 f.l. + VirB8 peri dom | pT7-7strep | ||
| StrepII-VirB4 f.l. + VirB11 f.l. | pT7-7strep | 1-831 and 1-361 | |
| VirBX Helicobacter pylori Cag | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| Pcag1 | TOPO T7/NT | 1-116* | |
| Pcag1 | TOPO T7/NT | 68-116* | |
| Pcag1 | pPROEXHTa | 1-116* | |
| pCag2 | TOPO T7/NT | 1-81* | |
| pCag2 | pPROEXHTa | 1-81* | |
| pCag3 | TOPO T7/NT | 1-482* | |
| pCag3 | pPROEXHTa | 1-482* | |
| pCag4 | TOPO T7/NT | 1-170* | |
| pCag4 | pPROEXHTa | 1-170* | |
| Pcag5 | TOPO T7/CT | 1-749* | |
| pCag6 | TOPO T7/NT | 1-200* | |
| pCag6 | pPROEXHTa | 1-200* | |
| Pcag7 | pPROEXHTb | 1496-1870 | (Virb10 region) |
| Pcag7 | pG6P | ||
| pCag8 VirB10 | TOPO T7/NT | 1-1327* | |
| pCag8 VirB9 | pPROEXHTa | 1-1327* | |
| pCag9 (27,28) | TOPO T7/NT | 1-536* | |
| pCag9 (29,30) | TOPO T7/NT | 27-249 | |
| pCag9 (31,28) | TOPO T7/NT | 453-536 | |
| pCag10 (33,34) VirB9 | TOPO T7/CT | 1-253* | |
| pCag10 (35,34) VirB9 | TOPO T7/CT | 58-353 | |
| pCag11 (37,38) | TOPO T7/NT | 1-219* | |
| pCag11 (37,40) | TOPO T7/NT | 1-100 | |
| pCag11 (37,38) | pPROEXHTa | 1-219* | |
| pCag13 | TOPO T7/NT | 1-197* | |
| pCag13 | TOPO T7/CT | 1-197* | |
| pCag13 | pPROEXHTa | 1-197* | |
| pCag14 | TOPO T7/NT | 1-127* | |
| pCag14 | pPROEXHTa | 1-127* | |
| pCag15 | TOPO T7/NT | 1-115 | |
| pCag16 | pPROEXHTa | 39-365 | |
| pCag17 | TOPO T7/CT | 1-307* | |
| pCag18 | TOPO T7/CT | 1-238* | |
| pCag19 | TOPO T7/CT | 1-382* | |
| pCag19 | pPROEXHTa | 126-381 | |
| pCag21 | pPROEXHTa | 1-143* | |
| pCag23 (67,68) CagE, VirB4 | TOPO T7/NT | 1-984* | |
| pCag23 (69,68) CagE, VirB4 | TOPO T7/NT | 101-984* | |
| Cag23, VirB4 C-domain | pET151 | 546-958 | |
| pCag25 | TOPO T7/CT | 1-116* | |
| pCag26 | TOPO T7/NT | 1-1187* | |
| HP0547/CagA | pPROEXHTb | 392-733 | |
| HP0533 (virD4?) | TOPO T7/NT | 1-30* | |
| HP0017 (Comb4) | pPROEXHTb | 34-336 | |
| pB7_Y24 virb7 (cag12, HP532) | pET15a | ||
| pB7_YN virB7 (cag12, HP532) | pET15a | ||
| Comb8 | |||
| ComB9 | |||
| HP0042 (Comb10) | pPROEXHTc | 1-233* | full length |
| ComB10 | |||
| HP0525 VirB11 | pPROEXHTc | 1-330* | |
| HP0525 VirB11 | pCDF-Duet-1 | 1-330* | |
| VirB11 | pET15a | full-length | |
| HP1451 | pPROEXHTa | 92-264 | |
| HP0524, VirD4 | pET14b | 170-end | |
| Agrobacterium tumefaciens | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| VirB4 | pET151 | 26-386 | domain N |
| VirB4 | pET151 | 390-759 | domain C |
| VirB4 | pET151 | 26-759 | Domain NC |
| VirB4 | pET151 | 173-375 | pfamN |
| VirB4 | pET151 | 1-789* | full length |
| VirB4 | pCDF | 1-789* | full length |
| VirB4 | pRSF | 1-789* | full length |
| VirB5 | pT7-7Strep | ? | full length |
| VirB8 | pT7-7strep | 73- | |
| VirB9 | pT7-7Strep | 22- | |
| VirB10 | pT7-7Strep | 51- | |
| VirB11 | pT7-7strep | ? | full-length |
| VirB11 | pET151 | 1-344* | full-length |
| VirB11 | pRSF | 1-344* | full-length |
| VirD4 | pET151 | 88-668 | DTMH |
| VirD4 | pCDF | 88-668 | DTMH |
| VirD4 | pRSF | 88-668 | DTMH |
| VirD4 | pET151 | 1-668* | full-length |
| Bartonella hensleae | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| VirB4 full length | pRS100 | * | full-length |
| virB1-B11 | genomic DNA | * | |
| Bartonella tribocorum | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| virB2-B11, virD4 | pAG11 | * | |
| trwD-N | cosmid pASB42-1 | * | |
| TrwK | pET151 | 90-361 | Domain N (B4) |
| TrwK | pET151 | 401-798 | Domain C (B4) |
| TrwK | pET151 | 90-798 | Domain NC (B4) |
| TrwK | pET151 | 401-786 | Domain Cs (B4) |
| TrwK | pET151 | 441-798 | Domain Cxs (B4) |
| TrwK | pET151 | 441-786 | Domain Cxxs (B4) |
| pKM101 of E. coli | |||
| Protein/construct | Vector | fragment aa (*is full length) | remark |
| pKM101 of E. coli | pKM101 | full plasmid | Remarks |
| TraL | pET101 | 1-245 | VirB1 full length C-ter His6 |
| TraB | pET151 | 1-442 | traB domain N (B4) |
| TraB | pET151 | 448-848 | traB domain C (B4) |
| TraB | pET151 | 1-848 | traB domain NC (B4) |
| TraB | pGEX-cs | 1-848 | GST-traB domain NC (B4) |
| TraB | pET151 | 1-866* | his_virB4 full length (actually traB) |
| TraB | pCDF | 1-866* | virB4 full length (actually traB) |
| TraB | pRSF | 1-866* | virB4 full length (actually traB) |
| TraB | pET101 | 1-866* | virB4_his full length (traB) |
| TraC | pET101 | 1-238 | VirB5 full length C-ter His6 (TraC) |
| TraC | pASK-IBA3C | 1-238 | VirB5 full length C-ter StrepII (TraC) |
| traD | pTrc200 | * | full length (B6) |
| TraE | pET151 | 1-257 | VirB8 full length N-ter His6 (TraE) |
| TraE | pCDF-Duet1 | 1-257 | VirB8 full length C-ter S-tag (TraE) |
| TraE | pCDF-Duet1/pRSF-Duet1 | 1-232 | VirB8 (TraE) N-ter His6-tag |
| TraE | pASK-IBA13 | 1-232 | VirB8 (TraE) N-ter StrepII (thrombin site) |
| TraE | pASK-IBA5C/pPR2 | 1-232 | VirB8 (TraE) N-ter StrepII |
| TraE | pASK-IBA6C/pASK-IBA12 | 70-232 | B8 (TraE) periplasmic N-ter StrepII tag |
| TraF | pET151 | 1-409 | VirB10 full length N-ter His6 (TraF) |
| TraF | pRSF-Duet1 | 1-409 | VirB10 full length N-ter His6 (TraF) |
| TraF | pCDF-Duet1 | 1-386 | VirB10 full length N-ter His6 (TraF) |
| TraF | IBA pASK13 | 1-386 | VirB10 (TraF) N-ter StrepII (thrombin site) |
| TraF | IBA pASK3C | 1-386 | VirB10 (TraF) N-ter StrepII tag |
| TraF | IBA pASK6C | 157-386 | B10(TraF) periplasmic N-ter StrepII tag |
| TraG | pET151 | 1-332* | his_traG full length (B11) |
| TraG | pCDF | 1-332* | traG full length (B11) |
| TraG | pRSF | 1-332* | traG full length (B11) |
| TraG | pET101 | 1-332* | virB11_his full length (traG) |
| TraJ | pET151 | 76-275 | his_virD4 DTMH (actualle traJ) |
| TraJ | pCDF | 76-275 | virD4 DTMH (actualle traJ) |
| TraJ | pRSF | 76-275 | virD4 DTMH (actualle traJ) |
| TraJ | pET151 | 1-275* | virD4 full length (actualle traJ) |
| TraJ | pCDF | 1-275* | virD4 full length (actualle traJ) |
| TraJ | pRSF | 1-275* | virD4 full length (actualle traJ) |
| TraJ | pET101 | 1-275* | VirD4_his full length (traJ) |
| TraN | pET101 | 1-49 | VirB7 full length C-ter His6 (TraN) |
| traN | pASK-IBA2C | 19-48 | pASK-IBA2c modified N-terminal His6 |
| TraN | pASK-IBA3C | 19-48 | pASK-IBA3c modified N-terminal His6 |
| traN | pET-Duet1 | 19-48 | pET modified N-terminal His6 |
| traN | pCDF-Duet1 | 19-48 | pCDF modified N-terminal His6 |
| TraN | pASK-IBA33 | 1-48* | full-length |
| TraN | pET30a | 1-48* | full-length |
| TraN | pCDF-Duet1 | 1-48* | full-length |
| TraN | pGEXcs | 19-48 | |
| TraO | pASK-IBA12 | 24-294 | lacking signal seq. aa24- |
| TraO | pASK-IBA13 | 24-294 | lacking signal seq. aa24- |
| TraO | pCDF-Duet1 | 24-294 | pCDF modified N-terminal strepII |
| TraO | pASK-IBA3c | 1-294* | full-length |
| TraO | pASK-IBA12 | 24-107 | N domain lacking signal seq. aa24- |
| TraO | pASK-IBA12 | 24-118 | N domain lacking signal seq. aa24- |
| TraO | pASK-IBA12 | 24-135 | N domain lacking signal seq. aa24- |
| TraO | pASK-IBA6C | 24-294 | lacking signal seq. aa24- |
| TraB+TraG | pCDF-Duet1 | 1-866 and 1-332 | VirB4+VirB11 full length (TraB+TraG) |
| TraE+TraG | pCDF-Duet1 | 1-257 and 1-332 | His6_VirB8+VirB11 full length (TraE+TraG) |
| TraF+TraG | pCDF-Duet1 | 1-409 and 1-332 | His6_VirB10+VirB11 full length (TraF+TraG) |
| TraE+TraF | pCDF-Duet1 | 1-232 and 1-386 | His6-VirB10+VirB8-Stag |
| Legionella pneumophila (Lvh) | |||
| genomic DNA | Legionella pneumophila strain JR32 | ||
| Genomic DNA (delta LVH) | Legionella pneumophila | strain LP02, thyA(-) and restriction (-) DELTA lvh system | |
| VirB4 | pCDF-Duet1 | 1-826 | LvhB4 full length N-ter His6 |
| VirB4 | pRSF-Duet1 | 1-826 | LvhB4 full length |
| VirB11 | pCDF-Duet1 | 1-345 | LvhB11 full length N-ter His6 |
| VirB11 | pRSF-Duet1 | 1-345 | LvhB11 full length N-ter His6 |
| VirB11 | pCDF-Duet1 | 1-345 | LvhB11 full length |
| Commercial Vectors | |||
| pGEX 6P1 | |||
| pAlter Ex2 | |||
| pMalgg | |||
| pMalg | |||
| pMal(BE) | |||
| pProEx Hta | |||
| pProEx Htb | |||
| pProEx Htc | |||
| pET151 TOPO | cytoplasmic TEV-cleavable N-term His-tag | ||
| pPR-IBA1 | C-ter StrepII tag | ||
| pPR-IBA2 | N-ter StrepII tag | ||
| pET101 TOPO | cytoplasmic uncleavable V5-His tag | ||
| pGEX-cs | GST | ||
| pET22b(+) (Novagen) | C-ter His6 tag | lepB signal sequence | |
| pET15b Novagen | Thrombin cleavable N-ter His6 tag | ||
| pCDFduet | N-ter His6tag (MCS1) and C-ter S-tag (MCS2) | ||
| pRSFduet | N-ter His6tag (MCS1) and C-ter S-tag (MCS2) | ||
| pASK-IBA2 | periplasmic non-cleavable C-term StrepII tag | ||
| pASK-IBA3C | C-ter StrepII tag | ||
| pASK-IBA4 | periplasmic non-cleavable N-term StrepII tag | ||
| pASK-IBA5C | N-ter StrepII tag | ||
| pASK-IBA6C | periplasmic factor Xa cleavable N-term StrepII tag | ||
| pASK-IBA7 | cytoplasmic factor Xa-cleavable N-term StrepII tag | ||
| pASK-IBA12 | periplasmic thrombin-cleavable N-term StrepII tag | ||
| pASK-IBA13 | cytoplasmic thrombin cleavable N-term StrepII tag | ||
| pASK-IBA32 | periplasmic C-terminal 6x-His tag | ||