Bonnie Ann Wallace

Professor of Molecular Biophysics
Institute of Structural and Molecular Biology
Birkbeck College
University of London
London WC1E 7HX U.K.

phone: 44-(0)207-631-6800
fax: 44-(0)207-631-6803
email: b.wallace@mail.cryst.bbk.ac.uk

Ph.D. (Molecular Biophysics and Biochemistry) Yale University
Jane Coffin Childs Postdoctoral Fellow, Harvard University
Jane Coffin Childs Postdoctoral Fellow, MRC-LMB, Cambridge
Fellow, AAAS; Fellow, Institute of Biology; Fellow, Royal Society of Chemistry; Fellow, IUPAC
2009 Royal Society of Chemistry Interdisciplinary Prize
2010 AstraZeneca Award from the Biochemical Society
2016 Honorary Member, British Biophysical Society

2017 Fellow, (US) Biophysical Society

Book on CD and SRCD:

"Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy"

B.A. Wallace and R.W. Janes, Editors
click here for information on contents and how to order

My lab focuses on structure/function studies of membrane proteins, especially those involved in ion translocation and transport. Of particular interest are voltage-gated sodium channels: we have determined the first crystal structure of a sodium channel in its open conformation . We have also determined the first crystal structures of complexes of a sodium channel with several eukaryotic sodium channel drugs, which we have shown (in collaboration with the Clapham Lab at Harvard) block prokaryotic channels, making this channel an excellent candidate for drug discovery/development, and we identified for the first time the locations of the sodium ions in the channel, and most recently determined the first structure of a full-length open activated sodium channel, which has revealed a novel interaction motif and has identified residues conserved across human and prokaryotic channels, that are associated with disease states. My lab uses the techniques of X-ray crystallography, cryoEM, circular dichroism spectroscopy, biophysical characterisation methods, cloning and expression, bioinformatics, molecular modelling and molecular dynamics, as well as functional studies to examine structure/function relationships for ion channels.

The other main focus of my lab is the development of techniques and applications for circular dichroism spectroscopy, including synchrotron radiation circular dichroism (SRCD) spectroscopy of proteins, and circular dichroism spectroscopy of membrane proteins, including ways of mitigating solvent shifts, differential scattering, and absorption flattening effects. We have created the DICHROWEB website for secondary structural calculations , new soluble and membrane protein reference databases for CD analyses, and a widely-used CD data processing, analysis and archive package "CDtool" for use with conventional CD and SRCD beamline data.

In a collaborative project with R.W. Janes of Queen Mary, University of London, we have created the Protein Circular Dichroism Data Bank (PCDDB), a deposition and archiving data bank and associated validation software for circular dichroism spectra and the DichroMatch method for structural analyses.

Selected Publications:

 

 

Our work is and has been supported by grants from the BBSRC, the EPSRC, the MRC, IUPAC, Pfizer Neusentis, Bayer Animal Health, and the Wellcome Trust.


I organise the EU/UK Annual CD Summer School. This year it will be held on 12 July 2017 at Warwick University. Details of the associated UK/EU Annual CD meeting can be accessed here.

 

Current Group Members

Postdocs: Dr. Lee Whitmore, Dr. Andrew Miles, Dr. Altin Sula

PhD Students: Alex Henderson, Elliot Drew, Matthew Colledge, Giulia Montini, Jenny Booker

MRes Student: Seolmin Ko

 

Previous Lab Members (Partial List with Current Institutions)

PhD Students: David Mao, Donna Mielke (Argnonne National Lab, USA), Michael Cascio (Duquesne University, USA), Nancy [Swords]Jenny (University of Vermont, USA), Diane Peapus, Manju Venugopal, Declan Doyle (Univ. of Southampton), Chris Snook, Mark Richards (Leeds University), Andrias O’Reilly (Liverpool John Moores University), Y-C Chen (McKay Medical College, Taiwan), Paul Evans, Jonathan Lees (University College London), Jasveen Chugh (UCB Pharma), Sharmeen Haque (University College London), Matthew Radford, Neil Freeman, Andrew Orry (MolSoft), Timothy Stone (University of Cardiff, Wales), Ali Abdul-Gader, Ben Woollett, Graciella Cid, Toby Galbraith, Andrew Miles (Birkbeck),Wayne Miller (GSK), Alix Blockley (Derby University)

Postdocs:  Drew Woolley (University of Toronto, Canada), K. Ravikumar (University of Madras, India), Martha Kimball, Kalypso Charalambous (Kings College London), Nora Cronin (Inst. Cancer Res.), Andrew Powl, Andrias O’Reilly (Liverpool John Moores Univ.), Ghasem Nurani, Emily McCusker (Teva Pharmaceuticals), Claire Bagneris (Birkbeck), Claire Naylor (Molecular Dynamics), Oliver Smart (EBI), Frank Wien (Soleil Synchrotron, France),

Jose Luiz Lopes (Univ. of Sao Paulo, Brazil), Juliana Yoneda (Univ. of Sao Paulo, Brazil), Geancarlo Zanetta (Univ. of Forteleza, Brazil), Martin Ulmschneider (Kings College London)