H52

Antibody Name: H52
References: Eigenbrot, C., Gonzalez, T., Mayeda, J., Carter, P., Werther, W., Hotaling, T., Fox, J. and Kessler, J. "X-ray structures of fragments from binding and nonbinding versions of a humanized anti-CD18 antibody: Structural indications of the Key role of VH residues 59-65" Proteins (1994) 18:49-62.
Donor Antibody: mouse
Acceptor Antibody: Human IgG1 | k
Antigen: B2-subunit (CD18) of integrins (LFA-1, Mac-1, P150,95)
Laboratory: Genentech, CA
Design: Common FR derived from consensus sequences of the most common human isotypes (VHIII, VkI).
Frameworks: VH SGIII | VL kI consensus sequences.
CDRs: All 6 VH and VL, Kabat Definition.
Backmutations: To be discussed in forthcoming publication by M. Zoller.
Binding: "Approaches" the affinity of the chimaeric mAb by competition binding. Best version h52-OZ.
Expression: E. coli
Comment: Structures show the importance of the C-terminal region of CDR-H2 (residues 59-65).
Clinical Indication: "Potential to ameliorate deleterious immunological and inflammatory processes".

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.