H17E2

Antibody Name: H17E2
References: Verhoeyen, M., Broderick, L., Eida, S. and Badley, A. "Re-shaped human anti-PLAP antibodies." In "Monoclonal Antibodies: Applications in Clinical Oncology" (1991) Ed. Epenetos A.A., Chapman & Hall Medical, London pp37-43.
Donor Antibody: mouse
Acceptor Antibody: Human IgG1 | k
Antigen: Human Placental Alkaline Phosphatase (PLAP) and testicular PLAP-like alkaline phosphatase but no cross-reactivity to liver or intestinal enzymes
Laboratory: Unilever, UK
Design: Mouse FR sequence similar to solved structure D1.3 Study all residue differences between mouse and human FRs with reference to structures of D1.3, NEW and REI. Most differences non-important on the Beta-sheet surface or far from CDRs.
Frameworks: VH human NEWM | VL human REI (modified as in Campath-1H.)
CDRs: All 6 from VH and VL, Kabat Definition.
Backmutations: VH S27F S30T as in Campath-1H
Binding: "Affinity (of humanised) ... reduced somewhat compared to H17E2". Dilution curves of mouse and humanised look similar.
Expression: mouse NS0 myeloma cells
Comment: Used to image ovarian cancer indicating in vivo binding. Source sequence supplied by Dr. Mahendra Deonarain.
Clinical Indication: Treatment of ovarian and testicular cancer.
See Hird et al (1991) Br. J. Cancer 64:911-914.

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.