Fd138-80

Antibody Name: Fd138-80 (IgG SGII | k SGV)
Reference: Co, M.S., Deschamps, M., Whitley, R.J. and Queen, C.
"Humanized antibodies for antiviral therapy."
Proc. Natl. Acad. Sci. USA (1991) 88: 2869-2873.
(See also mAb Fd79 in this reference).
Donor Antibody: mouse
Acceptor Antibody: Human IgG1 | k
Antigen: Herpes simplex virus (HSV) gD glycoprotein
Laboratory: Protein Design Labs, CA; Univ. Alabama, AL
Design: As for Anti-Tac.
Frameworks: VH human EU | VL human EU
CDRs: All 6 from VH and VL, Kabat Definition.
Backmutations: VH G27Y S30T M48F R66K V67A | VL Y36H (structural model suggests contacts with CDRs)
VH V37M | VL Y87F (model suggests contact with F98:L adjacent to CDR)
VH F89V G94R E103W Y104G N105Q G107T | VL M48I I63T (atypical amino acids, backmutations consistent with human consensus)
Binding: The humanised mAb's affinity is "comparable" to the mouse mAb as assessed by competition binding.
Expression: Sp2/0 mouse myeloma cells
Comment: Assays showed the humanised mAb had neutralising activity for HSV-1

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.