Antibody Name: DX48
References: Lewis, A.P. and Crowe, J.S. "Immunoglobulin complementarity-determining region grafting by recombinant polymerase chain reaction to generate humanised monoclonal antibodies." Gene (1991) 101: 297-302.
Donor Antibody: rat
Acceptor Antibody: Human IgG1 | rat VL
Antigen: Digoxin
Laboratory: Wellcome Research Labs, UK
Design: New approach to rapidly generate humanised mAbs by CDR-grafting using polymerase chain reaction (PCR).
Frameworks: VH NEWM from Campath-1H
CDRs: All 3, but only CDR-H1 in this experiment, Kabat Definition.
Backmutations: F27S:H T30S:H (from Campath-1H to NEW again)
Binding: No data given
Expression: No data given
Comment: Source sequences supplied by Dr. Scott Crowe.
Clinical Indication: Of academic interest only.

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.