D1.3

Antibody Name: D1.3
Reference: Foote, J. and Winter, G. "Antibody framework residues affecting the conformation of the hypervariable loops" J. Mol. Biol. (1992) 224:487-499.
Donor Antibody: mouse
Acceptor Antibody: Human IgG1 | k
Antigen: lysozyme
Laboratory: MRC, LMB, Cambridge, UK
Frameworks: VH human NEWM
VL kI consensus sequence (using Kabat Ed IV 1987) (same FR as CAMPATH-1H).
CDRs: All 6 VH and VL, Kabat Definition.
Backmutations: STFS27-30FSLT:H V71K:H F71Y:L
Binding: By fluorescence quenching, 4-fold reduction in affinity compared to the mouse mAb for the best version (HyLys11)
Expression: non-secreting mouse myeloma NS0
Comment: Study to dissect contribution of FR residues by "rebuilding" the affinity. Coined the phrase VERNIER zone; a layer of FR residues beneath the CDRs which support their conformation and/or relative disposition. See also Verhoeyen et al (1988) Science 239: 1534-1536.

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.