D1.3

Antibody Name: D1.3
Reference: Verhoeyen, M., Milstein, C. and Winter, G. "Reshaping human antibodies: Grafting an antilysozyme activity." Science (1988) 239: 1534-1536.
Donor Antibody: mouse
Acceptor Antibody: Human IgG2 | mouse k
Antigen: lysozyme
Laboratory: MRC, LMB, Cambridge, UK
Frameworks: VH human NEWM | VL mouse k
CDRs: All 3 VH, Kabat Definition.
Backmutations: none
Binding: By fluorescence quenching and competition binding assays, 10-fold lower binding than mouse IgG1
Expression: mouse J558L myeloma cells, λ light chain exchanged in vitro
Comment: "The result is remarkable given the several assumptions underlying the building of an antigen-binding site"
See also Foote & Winter (1992) J.Mol.Biol.224:487-499.

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.