|Antibody Name: ||
B1-8 (μ | λ)
Jones, P.T. , Dear, P.H., Foote, J., Neuberger, M.S. and Winter, G. "Replacing the complementarity-determining regions in a human antibody with those from a mouse." Nature (1986) 321: 522-525.
|Donor Antibody: ||
|Acceptor Antibody: ||
Human IgE | mouse Λ1
hapten NP-cap (4-hydroxy-3-nitrophenacetyl caproic acid) or NIP-cap
(3-iodo-4-hydroxy-5-nitrophenyl-acetyl caproic acid)
MRC, LMB, Cambridge, UK
VH human NEWM | VL mouse Λ1
All 3 from VH, Kabat Definition.
2/3-fold lower affinity than mouse mAb measured by fluorescence
quenching but less than that expected for loss of a hydrogen-bond
or van der Waals' contact.
stably transformed J558L cells
First example of 'CDR-replacement' to find out "whether the FRs
represent a simple B-sheet scaffold on which new binding sites may be
built and whether the structure of the CDRs (and antigen binding) is
therefore independent of the FR context."