B1-8

Antibody Name: B1-8 (μ | λ)
Reference: Jones, P.T. , Dear, P.H., Foote, J., Neuberger, M.S. and Winter, G. "Replacing the complementarity-determining regions in a human antibody with those from a mouse." Nature (1986) 321: 522-525.
Donor Antibody: mouse
Acceptor Antibody: Human IgE | mouse Λ1
Antigen: hapten NP-cap (4-hydroxy-3-nitrophenacetyl caproic acid) or NIP-cap (3-iodo-4-hydroxy-5-nitrophenyl-acetyl caproic acid)
Laboratory: MRC, LMB, Cambridge, UK
Frameworks: VH human NEWM | VL mouse Λ1
CDRs: All 3 from VH, Kabat Definition.
Backmutations: none
Binding: 2/3-fold lower affinity than mouse mAb measured by fluorescence quenching but less than that expected for loss of a hydrogen-bond or van der Waals' contact.
Expression: stably transformed J558L cells
Comment: First example of 'CDR-replacement' to find out "whether the FRs represent a simple B-sheet scaffold on which new binding sites may be built and whether the structure of the CDRs (and antigen binding) is therefore independent of the FR context."

explanation of symbols and abbreviations

José Saldanha © 1997-8. Birkbeck College, London WC1E 7HX.