Identify Putative Backmutations in the Chosen Framework
Reconsider Framework choice and Design Humanised Antibody Sequence
Construct Humanised and (if possible) Chimaeric Antibody Sequences. Utilise optimal codon usage from Kabat and use degenerate codons to create or destroy restriction sites.
Test Constructs
(Humanised light chain can be tested independently by combining with heavy Chimaeric chain and vice versa).
