The Design Cycle
Donor Amino Acid Sequences
the Fv/Constant Regions
Acceptor Framework Sequences
in the Chosen Framework
Reconsider Framework choice
and Design Humanised Antibody Sequence
Construct Humanised and (if possible) Chimaeric Antibody Sequences. Utilise optimal codon usage from Kabat and use degenerate codons to create or destroy restriction sites.
(Humanised light chain can be tested independently by combining with heavy Chimaeric chain and
Success? If "No" then Continue
Return to "Consider the Human Acceptor ..."
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