Electron microscopy and image processing

Birkbeck College
Crystallography, Department of Biological Sciences and ISMB

EM Lab 020 7079 8061
T12 Microscope ext 8057

T10 Microscope ext 8058
Polara 0203 0738 090 
BBKISMB logo

COPII coatTubulin-KinesinViral capsidFlexible fittingMACPF porePhage connector-tailDyneinGroEL trajctory



EM Lab Manager Post Available

EM lab safety


EM Wiki


Local EM information


Booking system

Helen Saibil
Carolyn Moores
Giulia Zanetti
Anthony Roberts
Natasha Lukoyanova
Natasha Dudkina
Katja Ota
Szymon Manka
Celia Deville
Helen White
Ottilie von Loeffelholz
Lyndon Newins
Ksenia Rhyzanova

Elena Orlova
Maya Topf

Julia Wenger
Natasha Burgess
Vicky Hale
Joe Atherton
Thanos Ignatiou
Tom Peskett
Francesca Coscia
Tiago Costa
Kat Toropova
Abid Javed

EMBO
                course

Course Files 2015
       

EM equipment
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Polara machines, with a K2 direct electron detector and Quantum energy filter on the Polara, DE20 direct detectors on the Polara and F20, and a vitrobot for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.

Image processing 
Hardware: Linux clusters and graphics workstations
Software: Spider, Imagic, MRC image programs, Relion, CCP4, EMAN, Amira, IMOD, Dynamo
Spider Imagic CCP4 CCP-EM EMAN IMOD RELION
Dynamo

eBIC: electron Bio-Imaging Centre at Diamond Light Source